Small RNA Library Prep Kit for Illumina (Indexes 25–48)

Library preparation kit for small RNA next-generation sequencing, optimized for low-input RNA samples including plasma, serum, and urine.

Product Overview

The Small RNA Library Prep Kit for Illumina (Indexes 25 -48) provides the reagents and components required to prepare small RNA libraries for next-generation sequencing (NGS) on compatible Illumina platforms. The kit includes molecular reagents such as adapters, primers, enzyme mixes, and buffers, along with a purification module for processing nucleic acid products generated during library preparation.

The workflow is designed for low-input RNA and is particularly suited to challenging sample types such as plasma and serum. According to the supplied product information, the kit can support library preparation from RNA inputs as low as 1 ng for specified sample types and can be completed in less than five hours, depending on the sample and workflow.

The purification module uses Norgen's resin-based technology to support recovery of library intermediates and final products. The workflow is intended for different input materials, including purified total RNA, enriched small RNA, and RNA isolated from liquid biopsy samples.

Key Features and Benefits

Low-Input RNA

Designed for low RNA quantities, with the supplied information specifying inputs as low as 1 ng for appropriate samples.

Streamlined Workflow

Library preparation can be completed in less than five hours according to the product description.

Liquid Biopsy Samples

Suitable for workflows involving RNA from plasma, serum, urine, and other compatible input materials.

Integrated Purification

Includes a purification module for recovering nucleic acid products generated during library preparation.

Research Applications

Small RNA sequencing enables researchers to investigate short RNA molecules and their roles in gene regulation, cellular processes, and disease-associated molecular changes. This kit may support research workflows such as:

Small RNA and microRNA sequencing studies.
Gene expression and post-transcriptional regulation research.
RNA profiling in plasma and serum samples.
Liquid biopsy research using compatible RNA isolated from biological fluids.
Biomarker discovery and comparative RNA expression analysis.
Next-generation sequencing library preparation for compatible Illumina systems.

General Library Preparation Workflow

Small RNA library preparation converts RNA molecules into sequencing-ready libraries. The exact procedure, reagent volumes, incubation conditions, and purification steps must follow the manufacturer's protocol.

Step 1: RNA Input

Prepare purified total RNA, enriched small RNA, or compatible low-input RNA.

Step 2: Library Construction

Use the kit's adapters, primers, enzymes, and buffers to construct small RNA libraries.

Step 3: Purification

Purify library intermediates and products using the supplied purification module.

Step 4: Sequencing Preparation

Assess library quality and prepare compatible libraries for sequencing according to the protocol.

Product Specifications

Product Name	Small RNA Library Prep Kit for Illumina (Indexes 25–48)
Catalog Number	1111-63620
Pack Size	24 preparations
Application	Small RNA library preparation for next-generation sequencing
Sequencing Platform	Illumina platforms compatible with the prepared libraries
Index Set	Indexes 25–48
Sample Types	Purified total RNA, enriched small RNA, plasma, serum, urine, and other compatible inputs
Low RNA Input	As low as 1 ng for specified sample types, according to the product description
Preparation Time	Less than 5 hours, according to the product description
Minimum Order Quantity	1
UNSPSC	41105905
UN Shipping Code	UN1845 (dry ice)
Shipping Conditions	Dry ice
Storage Conditions	Freezer
Storage Temperature	-20°C
Shelf Life	At least 1 year when stored under the specified conditions

Storage and Handling

Store the kit at -20°C, as indicated in the supplied product information. The reagents are stated to remain stable for at least one year when stored under the specified conditions. Follow the manufacturer's instructions for reagent handling, thawing, storage after opening, and freeze-thaw limitations.

Safety Information

The supplied listing identifies this product as hazardous and gives UN1845 as the UN shipping code, which refers to dry ice used for transport. Consult the current Safety Data Sheet (SDS) and applicable transport documentation to identify the relevant hazards and handling requirements for the kit itself. Use appropriate personal protective equipment and follow laboratory safety procedures.