Amplite® Fluorimetric Proteasome 20S Activity Assay Kit - Green Fluorescence

Fluorescent detection of 20S proteasome chymotrypsin-like activity in cultured cells and cell lysates.

Product Overview

The Amplite® Fluorimetric Proteasome 20S Activity Assay Kit is a homogeneous fluorescence-based assay designed to measure the chymotrypsin-like protease activity associated with the 20S proteasome complex. It can be used with cultured cells or cell lysates for research involving proteasome function and cellular protein degradation.

The green fluorescence detection format provides a convenient way to monitor proteasome-associated enzymatic activity. Fluorescence measurements can be used to compare activity between experimental samples when appropriate controls and validated assay conditions are applied.

Assay Principle

The 20S proteasome is a proteolytic complex involved in the breakdown of proteins inside cells. Its chymotrypsin-like activity represents one of the proteolytic activities commonly studied in proteasome research.

In a fluorimetric assay, proteasome-associated enzymatic activity acts on an appropriate assay substrate to generate a fluorescent signal. The resulting fluorescence is measured using a compatible fluorescence microplate reader or other suitable instrument, following the manufacturer's protocol.

Fluorescence intensity may be used to compare relative activity across samples. Results depend on sample preparation, assay conditions, instrument settings, and the appropriate use of controls.

Experimental Considerations

• Follow the manufacturer's instructions for sample preparation, reagent handling, incubation, and fluorescence measurement.
• Include appropriate blanks and controls to help distinguish assay signal from background fluorescence.
• Use consistent sample handling and measurement settings when comparing experimental groups.
• Review the product documentation for reagent storage requirements and compatibility with your samples and instruments.

Safety Information

The supplied product listing identifies the kit as non-hazardous for shipping purposes. Always consult the current Safety Data Sheet (SDS) and the manufacturer's instructions before use. Follow standard laboratory safety procedures and wear appropriate personal protective equipment.

Order Amplite® Proteasome 20S Activity Assay Kit

Explore product details and availability for the 100-test green fluorescence assay kit.

Catalog No.: 0271-13456-AAT | Size: 100 Tests