Product Information

Product name: Anti-C5a-R/C5ar1 Antibody Picoband®

Catalog number: 0519-A01898-2

Pack size: 100 µg/vial

Target: Complement component 5a receptor 1 (C5aR1)

Alternative names: C5a receptor, C5AR1, CD88

Reactivity: Mouse

Reported applications: ELISA, flow cytometry, immunofluorescence (IF), immunocytochemistry (ICC), Western blot (WB)

Antibody type: Research antibody

Dry ice: No

Hazardous: No, as listed

Log in to view pricing and ordering options.

Login for Price

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

What Is C5aR1?

Complement component 5a receptor 1 (C5aR1), also known as CD88, is a G protein-coupled receptor that recognizes C5a, a potent inflammatory mediator generated during activation of the complement system. C5aR1 is expressed by several types of immune cells and contributes to cellular responses involved in inflammation and immune defense.

When C5a binds to C5aR1, the receptor activates intracellular signaling pathways that can influence immune cell migration, activation, and the release of inflammatory mediators. These functions make C5aR1 an important subject of research into complement biology, inflammatory disorders, and immune regulation.

The Anti-C5a-R/C5ar1 Antibody Picoband® is described as reactive with mouse C5aR1 and is listed for several laboratory applications. Researchers can use a target-specific antibody to investigate receptor expression and localization in appropriately prepared samples.

Biological Role of the C5a Receptor

The complement system is part of innate immunity. During complement activation, the cleavage of complement component C5 generates C5a and C5b. C5a acts as a signaling molecule, while C5b participates in the formation of the membrane attack complex.

C5aR1 mediates many of the cellular effects of C5a. Its activation can contribute to:

• Immune cell recruitment: Supporting the movement of responsive immune cells toward sites of inflammation.
• Inflammatory signaling: Activating intracellular pathways that regulate immune cell activity.
• Neutrophil and myeloid cell responses: Modulating the activity of immune cells involved in host defense.
• Immune regulation: Influencing interactions between complement signaling and other immune pathways.
• Disease research: Providing a target for investigating the contribution of complement signaling to inflammatory and other pathological processes.

C5aR1 activity can have different effects depending on the biological context, cell type, and disease model. Its expression alone does not establish that the receptor is activated or that it causes a particular biological outcome.

Applications of Anti-C5aR1 Antibody

Western Blot (WB)

Western blotting separates proteins by electrophoresis before antibody-based detection. A C5aR1 antibody may be used to investigate the presence of the target protein in suitable mouse samples. Because C5aR1 is a membrane receptor, sample preparation and membrane protein extraction can influence detection. Confirm the expected band and conditions with product-specific validation data.

Flow Cytometry

Flow cytometry measures fluorescence associated with individual cells. An antibody suitable for flow cytometry may help researchers assess C5aR1 expression in mouse cell populations. For surface staining, the antibody must recognize an accessible extracellular epitope under the chosen staining conditions. Confirm whether the product has been validated for surface or intracellular detection.

Immunofluorescence (IF)

Immunofluorescence uses fluorescent detection to examine target distribution in cells or tissue samples. C5aR1 staining can support investigations of receptor localization, provided that sample preparation, antibody specificity, and detection conditions are appropriately controlled.

Immunocytochemistry (ICC)

Immunocytochemistry is used to detect proteins in cultured cells or cell preparations. A C5aR1 antibody may be used to investigate receptor distribution in mouse cells when compatible with the fixation, permeabilization, and detection protocol.

ELISA

Enzyme-linked immunosorbent assays use antibody-based recognition to detect or quantify a target. The supplied product listing reports ELISA as an application. Confirm the intended ELISA format and validated protocol before use, as not every antibody is suitable for every assay configuration.

Key Product Features

• Target-specific research: Directed against C5aR1/C5ar1, also known as CD88.
• Mouse reactivity: The supplied product description identifies mouse as the reactive species.
• Multiple listed applications: ELISA, flow cytometry, IF, ICC, and Western blot.
• Complement research: Useful for studies investigating the receptor involved in C5a-mediated signaling.
• Convenient pack size: Supplied as 100 µg per vial.

Technical Specifications

Experimental Considerations

• Confirm the antibody's recommended storage conditions and reconstitution instructions using the current product datasheet.
• Use appropriate positive and negative controls to assess staining specificity.
• Choose sample preparation and detection conditions suited to the selected application.
• For flow cytometry, verify whether the antibody is validated for cell-surface staining, intracellular staining, or both.
• For Western blotting, verify the expected molecular-weight range and interpretation guidance using product-specific validation data.
• Optimize antibody concentration and incubation conditions for the sample type and assay.

Frequently Asked Questions

What does C5aR1 stand for?

C5aR1 stands for complement component 5a receptor 1. It is also known as CD88 and is a G protein-coupled receptor involved in responses to the complement fragment C5a.

Which species does this antibody recognize?

The supplied product description reports reactivity with mouse.

Which applications are listed for this antibody?

The listed applications are ELISA, flow cytometry, immunofluorescence, immunocytochemistry, and Western blot. Confirm application-specific validation and protocols before beginning experiments.

Why is C5aR1 important in immunology research?

C5aR1 mediates cellular responses to C5a and is involved in complement-associated inflammatory signaling. It is studied to understand immune cell activity and the contribution of complement pathways to disease processes.