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Enzyme Used in PCR: Complete Scientific Guide to DNA Amplification What is the Enzyme Used in PCR? The enzyme used in PCR (Polymerase Chain Reaction) is a DNA polymerase responsible for synthesizing new DNA strands during amplification. The most commonly used enzyme is Taq DNA polymerase, a heat-stable enzyme that can withstand the high temperatures required during PCR cycling. This enzyme is essential for copying specific DNA sequences exponentially, making PCR one of the most powerful techniqu …