T4 RNA Ligase 1
RNA & DNA MODIFYING ENZYMES
T4 RNA Ligase 1
RNA Ligation Enzyme for Molecular Biology and RNA Research
T4 RNA Ligase 1
A molecular biology enzyme used for joining RNA molecules through phosphodiester bond formation.
| CAT# | 0806-RK20503-01 |
| Size | 1000 U |
| Dry Ice | No |
| Hazardous | No |
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product Description
T4 RNA Ligase 1 is an ATP-dependent enzyme involved in RNA end joining. It catalyzes the formation of phosphodiester bonds between compatible RNA termini, joining a 5′-phosphate group to a 3′-hydroxyl group.
This enzyme is widely used in molecular biology workflows involving RNA modification, RNA labeling, adapter ligation, and RNA library preparation. Its ability to join RNA molecules makes it a valuable tool for researchers working with RNA structure, RNA processing, and molecular analysis.
T4 RNA Ligase 1 is particularly useful in applications requiring the ligation of single-stranded RNA molecules.
How Does T4 RNA Ligase 1 Work?
T4 RNA Ligase 1 facilitates RNA ligation through an ATP-dependent enzymatic reaction. The process involves activation of the enzyme, transfer of an AMP group to the RNA substrate, and formation of a phosphodiester bond between compatible RNA ends.
5′-phosphate end
3′-hydroxyl end
Phosphodiester bond formation
Schematic representation of RNA end joining.
Applications of T4 RNA Ligase 1
T4 RNA Ligase 1 supports several RNA-related molecular biology applications.
RNA Adapter Ligation
Joining RNA adapters to RNA molecules during preparation of sequencing libraries.
RNA Sequencing
Supporting RNA library construction workflows that require RNA end ligation.
RNA Labeling
Used in enzymatic RNA labeling strategies and molecular analysis.
RNA Modification
Facilitating RNA end modification and joining in biochemical research.
Small RNA Research
Supporting workflows involving small RNA molecules and RNA analysis.
Molecular Biology
Useful for investigating RNA structure, processing, and molecular interactions.
Key Product Features
- ATP-dependent RNA ligation activity.
- Catalyzes phosphodiester bond formation between compatible RNA ends.
- Useful for single-stranded RNA ligation workflows.
- Supports RNA adapter ligation and RNA library preparation.
- Suitable for molecular biology and RNA research applications.
- Supplied in a 1000 U size.
Technical Specifications
| Product Name | T4 RNA Ligase 1 |
| Catalog Number | 0806-RK20503-01 |
| Quantity | 1000 U |
| Product Category | DNA or RNA Ligases |
| Application | RNA Ligation |
| Dry Ice | No |
| Hazardous | No |
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