ExoQuick Plasma Prep and Exosome Precipitation Kit (75 Reactions)

ExoQuick Plasma Prep and Exosome Precipitation Kit (75 Reactions)

Product Overview

The ExoQuick Plasma Prep and Exosome Precipitation Kit is a high-performance solution designed for rapid and efficient isolation of exosomes from plasma samples. This kit combines ExoQuick reagent (5 mL) with Thrombin (500 µL at 500 U/mL) to enable both plasma preparation and exosome precipitation in a streamlined workflow.

Engineered for reproducibility and ease of use, this kit allows researchers to isolate intact extracellular vesicles (EVs), including exosomes, without the need for ultracentrifugation or specialized equipment.

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Key Features

  • Complete workflow solution: Plasma preparation + exosome isolation in one kit
  • High yield and purity: Efficient precipitation of exosomes from plasma
  • No ultracentrifugation required: Simple benchtop protocol
  • Optimized for plasma samples: Includes thrombin for fibrin removal
  • Scalable: Suitable for up to 75 reactions
  • Preserves vesicle integrity: Ideal for downstream molecular analysis

Kit Components

  • ExoQuick Solution (5 mL)
  • Thrombin (500 µL at 500 U/mL)
  • User protocol

Principle of the Assay

The kit operates via a polymer-based precipitation method, where the ExoQuick reagent reduces the solubility of exosomes, allowing them to be pelleted by low-speed centrifugation.

Thrombin is first used to treat plasma samples to remove fibrin, ensuring cleaner downstream isolation. Following this, exosomes are precipitated efficiently without compromising their structural integrity or biological activity.

Applications

  • Exosome isolation from plasma
  • Biomarker discovery
  • Liquid biopsy research
  • RNA and protein profiling
  • Cancer diagnostics research
  • Cell-to-cell communication studies

Advantages Over Ultracentrifugation

Feature ExoQuick Kit Ultracentrifugation
Time required < 2 hours 4–24 hours
Equipment Standard centrifuge Ultracentrifuge required
Yield High Variable
Ease of use Simple Complex
Reproducibility High Moderate

Technical Specifications

  • Sample type: Plasma
  • Reactions: 75
  • Format: Liquid reagents
  • Storage: As per manufacturer instructions
  • Processing time: ~1.5–2 hours

Protocol Overview

  1. Treat plasma with Thrombin to remove fibrin
  2. Centrifuge to clarify the sample
  3. Add ExoQuick solution
  4. Incubate (typically 30 minutes)
  5. Centrifuge to pellet exosomes
  6. Resuspend for downstream applications

Why Choose This Kit?

This kit is ideal for laboratories seeking a fast, reliable, and scalable solution for exosome isolation from plasma. Its simplicity makes it suitable for both routine workflows and high-throughput studies, while maintaining the quality required for advanced molecular analyses.