OptiPrep™ The optimum density gradient medium
OptiPrep™
The optimum density gradient medium
OptiPrep™ is a sterile and endotoxin tested solution of 60% iodixanol in water with a density of
1.32 g/ml
OptiPrep™ is a sterile and endotoxin-tested solution of 60% iodixanol in water with a density of 1.32 g/mL.
Iodixanol was developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing. It is non-ionic, non-toxic to cells, and metabolically inert. Iodixanol solutions can be made isoosmotic at all useful densities. These solutions also have low viscosity and osmolality.
The high density of OptiPrep™ facilitates the fractionation of cells by flotation from a dense load zone through either a continuous or discontinuous gradient, or through a simple density barrier.
Applications
Cell isolation
51 protocols available
Improved resolution of cell organelles
Low viscosity, isoosmotic gradients provide rapid and efficient separation of the major organelles in preformed gradients
Subcellular organelles and membranes
62 protocols available
Ideal for virus purification
Viruses purified using OptiPrep™ gradients show infectivity:particle number ratios at least 100× those from CsCl gradients
Virus purification
38 protocols available
Macromolecules and lipoproteins
13 protocols available
Specifications
OptiPrep™ is a ready-made, sterile, and endotoxin-tested solution with the following specifications:
Iodixanol: 60% (w/v)
Density: 1.320 ± 0.001 g/mL
Endotoxins: <1.0 EU/mL
Each batch of OptiPrep™ is checked for endotoxins using a specific LAL test. Our goal is to produce batches with an endotoxin level ≤0.13 EU/mL.
For every batch produced, a Certificate of Analysis showing the actual values of density and endotoxin levels is available at www.axis-shield-density-gradient-media.com. OptiPrep™ is manufactured, packed, and released in compliance with ISO 13485 and sterility according to Ph.Eur.
Package size: Prod. no. 1114542 – 1×250 mL
Ideal For
Mammalian and non-mammalian cells
Subcellular organelles
Analysis of membrane trafficking and cell signalling
Endocytosis and exocytosis studies
Plasma membrane and domains
Membrane vesicles and cytosol
Organelles from non-mammalian sources
Viruses
Plasma lipoproteins
Proteins and protein complexes
Plasmid DNA
Ribonucleoproteins