InstantBlue - 1L

InstantBlue - 1L

Overview

InstantBlue™ (1L) is a ready-to-use, ultra-fast Coomassie-based protein stain designed for rapid visualization of proteins in SDS-PAGE gels. It delivers clear, high-contrast blue bands within minutes using a simple one-step protocol without the need for fixing, washing, or destaining.

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Key Features

  • Ultra-fast staining: Visible protein bands in 5–15 minutes
  • One-step protocol: No fixation, washing, or destaining required
  • High sensitivity: Detects as little as ~5 ng of protein per band
  • Low background: Produces sharp bands with excellent signal-to-noise ratio
  • Non-toxic formulation: Methanol- and acetic acid-free for safer handling
  • Mass spectrometry compatible: Suitable for downstream protein analysis

Applications

  • SDS-PAGE protein visualization
  • Protein expression and purification analysis
  • Western blot workflow validation
  • Quantitative densitometry studies

Protocol (Simplified)

  1. Remove gel after electrophoresis
  2. Immerse directly in InstantBlue stain
  3. Incubate ~15 minutes at room temperature
  4. Visualize bands immediately (no destaining required)

 

     Product Details

    • Format: Ready-to-use solution
    • Volume: 1 Liter
    • Storage: Room temperature or 2–8°C (depending on supplier)
    • Capacity: ~40 mini gels per bottle

     Advantages Over Traditional Coomassie Staining

    • No overnight destaining
    • Faster workflow (minutes vs hours)
    • Reduced handling steps → lower variability
    • Cleaner gels with minimal background